Method for validating sterilization methods with two consecutive contaminations

摘要

The invention relates to a method for verifying a sterilization method of an article to verify the sterility assurance level obtained by the sterilization method. The method of the invention is characterized in that the container (12) receiving the product is polluted in the first stage, which is composed of more than 10 living microbial cells, and then disinfected in the first stage by the method. Then the container (12) is opened, contaminated by more than 10 living microorganisms, and the second sterilization cycle is carried out in the same way.Finally, check the sterility (12) of the container after the first and second cycles. It is especially suitable for health products and devices.