Method for the culture of nematodes of Rhabditis sp. genus and determination of the nematocidal substances activity

摘要

the object of the application is the farming method of nematodes, which is that in the medium containing agar and aqueous solution of bovine serum 70 - 90% used in proportions by weight from 1: 3 to 1: 7good 1: 5, applied nematodes of rhabditis sp.in the form of a suspension in physiological saline solution 0.6% nacl in standard quantities for breeding and production is at 18 - 30 oc 21°c favourably for a period of 4 - 7 dayspreferably 5 - 6 daysthen so obtained breeding shall be as nicieniobójczym substances of natural or synthetic origin and incubated in the disappearance of the death of nematodesafter incubation with agarowego nematodes with solid substrate using 0.6% nacl, and turns methylene blue and / or blue trypanu,and is then analysed by microscopy and quantitative content persistent individuals. positive when cultured nematode counts using the burkera.