Method for increasing activity and stability of laccase using the phosphorized buffer

摘要

The purpose of the notification is to increase the activity and stability of the fungal lacquer obtained by liquid cultures of Trametes versicolor on substrate PD containing 5 g/l potato extract, 10 g/l glucose with the addition 0,1 g/l MgSO4, 0,6 g/l KH2PO4; pH 6,0, which is kept for ten days at a temperature of 25oC, with a water solution of CuSO4 (1 mM/l) added to the farm on the 7-day incubation;and i s filtered after completion of the culture to separate the fungus from the chilled medium and subjected to a purification process using the TPP method, ion chromatography and molecular saturation technique and the resulting lacase is diluted in the phosphate buffer pH 6,0 in relation to 1:50 v/v is that the lacquer obtained is exposed to a rotating magnetic field, with a frequency of 30 Hz rotation, magnetic induction 17,5 mT, at a temperature 30-to-0,5-c and within eight hours.