In the presence of active and stable organic solvents in detergent, the preparation, purification and biochemical characteristics of a novel acid and heat-resistant SPPs protease serine produced by tolerant pleurisy strain ctm10057 were studied.

摘要

The present invention relates to uniform purification and biochemical and molecular characteristics of alkaline serine protease produced by strain ctm10057 of sajor caju. The molecular weight of the purified enzyme was estimated to be 65 000 to 10% per SDS PAGE. The sequencing of NH2 terminal also confirmed the purity of the enzyme. He showed a cherryDo not be homogenous with fungal serine protease. The optimal protease activity was obtained at pH 9.5 and 70 ° C. SPPs was completely inhibited by PMSF and DFP D. SPPs belongs to serine protease family. The range of pH (- 6-12) and high temperature (- 80-100 ℃) is very wide. Its thermal sensitivity and thermal stability are considered to be abl.Improved from 2 mM calcium The serine protease can be used as a biological additive in detergent preparation. In the presence of organic solvents (50% v / / V), especially chloroform, it is a stable peptide synthesis candidate. In the presence of SDS surfactant, SPPs enzyme also had significant stability.Surfactants (Tween 20, Tween 80 and Triton X-100)2.（Perborate de sodio et h lt sub gt 2 lt；/ In addition, SPS protease was added to detergent solution to improve the performance of dixan detergent to improve the bleaching of blood pollutants. In addition, the specificity of SPPs is that it has a wide range of specificity for protein matrix, and compared with commercial protease, it has better catalytic efficiency: pyrolytic protein X type and flavuric acid 500 L. in addition, the SPPs gene encoding SPS protease was cloned,The sequence and expression of recombinant protein (RSPS) expressed in extracellular space in E. coli BL21 plys s had the same characteristics as native protein (SPS). Therefore, SPPs protease seems to satisfy most of the good proteases that provide the synthesis of PID and biological additives in liquid and solid detergent formulations that are stable under industrial conditions.