PLASMA-BASED DETECTION OF ANAPLASTIC LYMPHOMA KINASE (ALK) NUCLEIC ACIDS AND ALK FUSION TRANSCRIPTS AND USES THEREOF IN DIAGNOSIS AND TREATMENT OF CANCER

摘要

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 1111111101111011101010111110101111101110111111110111011111111001111101111011111 International Bureau 0.. .... .. (10) International Publication Number (43) International Publication Date ...... ... WO 2017/181183 Al 19 October 2017(19.10.2017) WIPO I PCT (51) International Patent Classification: (74) Agents: KARNAKIS, Jennifer A. et al.; COOLEY LLP, C12Q 1/68 (2006.01) Attn: Patent Group, 1299 Pennsylvania Avenue, NW, Suite 700, Washington, District of Columbia 20004 (US). (21) International Application Number: PCT/US2017/027944 (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (22) International Filing Date: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, 17 April 2017 (17.04.2017) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (25) Filing Language: English DO, HN, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (26) Publication Language: English KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (30) Priority Data: MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, 62/322,982 15 April 2016 (15.04.2016) US NI, RU, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (71) Applicant: EXOSOME DIAGNOSTICS, INC. [US/US]; TH, Riverside Technology Center, 840 Memorial Drive, Suite ZA, ZM, ZW. 3, Cambridge, Massachusetts 02139 (US). TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (84) Designated States (unless otherwise indicated, for every (72) Inventors: SKOG, Johan Karl Olov; c/o Exosome Dia- kind gnostics, Inc., 840 Memorial Drive, Suite 3, Cambridge, GM, Massachusetts 02139 (US). NOERHOLM, Mikkel; c/o TZ, of regional protection available): ARIPO (BW, GH, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Exosome Diagnostics GmbH, Am Klopferspitz 19a, 82152 TJ, Martinsried (DE). BRINKMAN, Kay; c/o Exosome Dia- DK, _ TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, — _ gnostics, Inc., 840 Memorial Drive, Suite 3, Cambridge, LV, Massachusetts 02139 (US). CASTELLANOS-RIZAL- SM, DOS, Elena; c/o Exosome Diagnostics, Inc., 840 Memori- GW, KM, ML, MR, NE, SN, TD, TG). al Drive, Suite 3, Cambridge, Massachusetts 02139 (US). MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, = HURLEY, James; c/o Exosome Diagnostics, Inc., 840 Published: Memorial Drive, Suite 3, Cambridge, Massachusetts 02139 — with international search report (Art. 21(3)) (US). = = = = = = = = = 1-1 M GC Il Il GC Il IN Il © (54) Title: PLASMA-BASED DETECTION OF ANAPLASTIC LYMPHOMA KINASE (ALK) NUCLEIC ACIDS AND ALK FU- " SION TRANSCRIPTS AND USES THEREOF IN DIAGNOSIS AND TREATMENT OF CANCER C ) .,.. (57) : The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from biological samples, including plasma samples.