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Multiple application approach for the photometric determination of an analyte in a fluid sample in an automated analyzer
Multiple application approach for the photometric determination of an analyte in a fluid sample in an automated analyzer
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机译:在自动分析仪中光度测定流体样品中分析物的多种应用方法
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摘要
Procedure for determining the amount of the analyte specific to a sample that may present interference in photometric assays, in which the specific analyte is quantified from the change in the optical signal of the reaction mixture after the interaction of the analyte with reagents of analyte-specific assays, comprising the steps of: a) generating multiple calibration curves for multiple wavelengths for the specific analyte of a sample to be determined, in which - calibration curve 1 is recorded at wavelength 1 and optimized reaction time 1 for samples that show no interference, - calibration curve 2 is recorded at wavelength 2 and reaction time 2 optimized for samples showing haemolytic interference, - calibration curve 3 is recorded at wavelength 3 and reaction time 3 optimized for samples showing icteric interference, - the calibration curve 4 is recorded at wavelength 4 and reaction time 4 for samples showing lipemic interference, - and depositing these predetermined measurement results in a data management system of the instrument platform, b) performing a interference test, simultaneously with the determination of the specific analyte, to quantify the quantity of interfering substances present in the sample to be determined and compare the quantity of each interfering substance with the predetermined cut-off values, c) measure the optical signal for the specific analyte of a sample to be determined in the reaction mixture at multiple wavelengths and time points, d) selecting the corresponding calibration curve of step a) depending on the interfering substances, e) quantifying the amount of the specific analyte of a sample to be determined by comparison with the calibration curve selected from step d) for the s chosen wavelengths.
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