A METHOD OF QUANTITATIVE DETERMINATION OF SARCOSINE IN A BIOLOGICAL SAMPLE USING ANTI-SARCOSINE ANTIBODIES AND PEROXIDASE-ACTIVE GOLD NANOPARTICLES OR QUANTUM DOTS

摘要

The invention deals with a method of quantitative determination of sarcosinein a biologicalsample with a sensitivity from 0.05 µM to 1.00 µM by means of anti-sarcosine antibodies andperoxidase-active gold nanoparticles or quantum dots, applying the ELISA andLFIA methods.It is based on the usage of immunised antibodies that specifically distinguishsarcosine. Thesubject matter of the invention is also a diagnostic strip for thedetermination by means of theLFIA method, which is designed for qualitative and quantitative determinationof sarcosine ina biological sample. It is convenient for a routine determination of sarcosinein diagnosticlaboratories, which may be performed within 2-3 hours, utilising equipmentcommonlyavailable in such laboratories, and it may be also used for self-diagnostics.