MICROARRAY BASED MULTIPLEX PATHOGEN ANALYSIS AND USES THEREOF

摘要

Provided herein is a three-dimensional lattice microarray system for DNA sequence detection and analysis and kits including components of the same. In the system a solid support has a plurality of bifunctional polymer linkers, optionally with a fluorescent label, each with a top domain and a bottom end attached thereto at the bottom end and a plurality of nucleic acid probes attached to the top domain of the plurality of bifunctional polymer linkers. Also provided is a method for fabricating the microarray system and methods to detect and identify pathogens in a plant sample or to determine copy number of a pathogen DNA in an unpurified nucleic acid sample by using a known copy number of synthetic DNA as an internal standard.