STANDARD LIBRARY OF LOW-MOLECULAR-WEIGHT HEPARIN, DALTEPARIN SODIUM, AND PREPARATION METHOD THEREOF

摘要

The present invention provides a method for preparing a standard library of a low-molecular-weight heparin, dalteparin sodium, and a product thereof. The method uses crude heparin sodium as a raw material and is based on compound enzymatic hydrolysis and a modified nitrite degradation method. After the steps of enzymatic hydrolysis, oxidation, purification by ultrafiltration, purification by ethanol precipitation, degradation, reduction, alkaline oxidation and purification, refining by ultrafiltration, freeze drying, etc., a standard library of a low-molecular-weight heparin, dalteparin sodium, with a particular average molecular weight can be prepared.