A NOVEL BIOREACTOR FOR MASS PRODUCTION OF ARBUSCULAR MYCORRHIZAL FUNGI

摘要

The present invention provides a novel bioreactor to maximize aseptic mass production and up scaling of AM fungi by cultivating the fungal spores and roots in a transformed hairy root of plant. The bioreactor includes a steel or glass, misting forming device, a special type of inoculation assembly, culture holding mesh and mist controlling system wherein liquid nutrient medium steadily floods in a form of mist inside the vessel and then drains out and recycles at regular intervals. Present invention provides novel, improved systems/apparatus for the growth and cultivation of AM Fungi affording efficient oxygen, nutrient distribution capacity throughout the reactor vessel, and provides improved oxygen, nutrient supply at low shear to prevent damage of shear-sensitive tissues of transformed hairy root to produce AM fungal spores which are contamination free, simple, inexpensive and effective using the novel bioreactor.