METHODS OF SUPPRESSING ADAPTOR DIMER FORMATION IN DEEP SEQUENCING LIBRARY PREPARATION

摘要

Disclosed are methods of suppressing adaptor dimer formation comprising: providing a target polynucleotide with a 5' and 3' end; providing a double stranded DNA adaptor with a 5' end and a 3' end that have sequence complementary to each other, ligating the double stranded adaptor to the target polynucleotide to form a ligation product. Also provided is a method of preparing a library of nucleic acid sequences comprising: providing a double-stranded DNA adaptor with 5' and 3' ends having a sequence complementary to each other, contacting the adaptor with a target nucleic acid sequences having a 5' and a 3' end, and ligating the adaptor with complementary sequence to the 5' and 3' ends of the target nucleic acid sequence using a double stranded DNA ligase. The disclosure also provides kits for suppression of adaptor dimer formation in deep sequencing containing a double stranded DNA adaptor with 5' and 3' ends having a sequence complementary to each other, suitable enzymes, buffers, dNTPS, etc.