首页> 外国专利> METHOD FOR MANUFACTURING BLENDED FILAMENT YARN FOR CELL CULTURE SUPPORT, BLENDED FILAMENT YARN FOR CELL CULTURE SUPPORT REALIZED THERETHROUGH, AND FABRIC COMPRISING SAME

METHOD FOR MANUFACTURING BLENDED FILAMENT YARN FOR CELL CULTURE SUPPORT, BLENDED FILAMENT YARN FOR CELL CULTURE SUPPORT REALIZED THERETHROUGH, AND FABRIC COMPRISING SAME

机译:制造用于细胞培养的混合长丝纱,用于实现细胞再生的混合的长丝纱的方法以及构成相同的织物

摘要

Provided is a method for manufacturing a blended filament yarn for a cell culture support. The method for manufacturing a blended filament yarn according to one embodiment of the present invention is performed by comprising the steps of: (1) preparing a first yarn having at least one of a first motif and a first motif conjugate having an amino acid sequence in which a first amino acid sequence motif is repeated at least twice, and a second yarn having at least one of a second motif, which is different from the first motif, and a second motif conjugate having an amino acid sequence in which a second amino acid sequence motif is repeated at least twice, respectively; and (2) plying the prepared first yarn and second yarn. According to this, different motifs are provided in a unitary blended filament yarn, and the blended filament yarn is very suitable for fully expressing each function of the motifs. Also, such a unitary blended filament yarn may make it possible to implement a micro environment in which the steps of attachment, migration, proliferation, and differentiation of cultured cells can take place continuously. Furthermore, it is possible to culture cell aggregates formed of cells with the same differentiation step, and at the same time to culture multiple cell aggregates with different differentiation steps through a unitary blended filament yarn. In addition, it is possible to three-dimensionally grow the cultured cells to be more suitable for application for in vitro experimental models or implantation in animal bodies, and thus the present invention may be widely applied to various products used in the field of cell culturing or tissue engineering such as a bioreactor, a cell culture container, an implantation kit, and the like.
机译:提供了一种用于细胞培养载体的混合长丝纱线的制造方法。根据本发明的一个实施方案的混合长丝纱线的制造方法包括以下步骤:(1)制备具有第一基序和具有氨基酸序列的第一基序​​缀合物中的至少一个的第一纱线。第一氨基酸序列基序重复至少两次,第二纱线具有至少一个与第二基序不同的第二基序,第二基序与第一基序不同,第二基序缀合物具有氨基酸序列,其中第二氨基酸序列基序分别重复至少两次; (2)将准备好的第一纱线和第二纱线合股。据此,在一体的混纺长丝纱线中提供了不同的图案,并且混纺长丝纱线非常适合于充分表达图案的每种功能。同样,这种单一的混合长丝纱线可以实现微环境,在该环境中培养细胞的附着,迁移,增殖和分化步骤可以连续进行。此外,有可能以相同的分化步骤培养由细胞形成的细胞聚集体,并同时通过单一的混合长丝纱线以不同的分化步骤培养多个细胞聚集体。另外,可以使培养的细胞三维生长以更适合用于体外实验模型或动物体内的植入,因此本发明可以广泛应用于细胞培养领域中使用的各种产品。或组织工程,例如生物反应器,细胞培养容器,植入套件等。

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