PRIMER SET, KIT, AND METHOD FOR DETECTING PORCINE DELTACORONAVIRUS

摘要

#$%^&*AU2019100972A420191003.pdf#####ABSTRACT The present invention discloses a primer set, a kit, and a method for detecting porcine deltacoronavirus (PDCoV), and relates to the field of viral molecular biological detection. The primer set provided by the present invention includes an upstream primer, a downstream primer, and a probe. The sequence of the upstream primer is depicted in SEQ ID NO. 1, the sequence of the downstream primer is depicted in SEQ ID NO. 2, and the sequence of the probe is depicted in SEQ ID NO. 3. Using the primer set of the present invention, the lower limit of detection (LLD) of the PDCoV is 26.6 copies/pL, featuring strong specificity, high sensitivity, and good between-run and within-run reproducibilities. One hundred and twenty clinical porcine fecal samples were assayed by the present invention, and the result showed a PDCoV positive rate of 25%.