首页> 外国专利> A HIGHLY ERROR-PRONE ORTHOGONAL DNA REPLICATION SYSTEM FOR TARGETED CONTINUOUS EVOLUTION IN VIVO

A HIGHLY ERROR-PRONE ORTHOGONAL DNA REPLICATION SYSTEM FOR TARGETED CONTINUOUS EVOLUTION IN VIVO

机译：用于体内有目标连续进化的高度错误消息的正交DNA复制系统

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摘要

The invention provides compositions comprising highly error-prone polymerases and methods of using the polymerases for rapid evolution of a nucleic acid sequence within host cells. The invention further provides a versatile synthetic biology platform for manipulating DNA replication inside a cell. The invention also provides a mutually orthogonal replication system for manipulating and tuning DNA replication of multiple nucleic acid molecules using the error-prone polymerases. 展开▼

机译：本发明提供了包含高度易错的聚合酶的组合物，以及使用该聚合酶在宿主细胞内快速进化核酸序列的方法。本发明进一步提供了用于操纵细胞内DNA复制的通用合成生物学平台。本发明还提供了使用易于出错的聚合酶来操纵和调节多个核酸分子的DNA复制的相互正交的复制系统。展开▼

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公开/公告号WO2019079775A3

专利类型

公开/公告日2019-06-06

原文格式PDF

申请/专利权人 THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;
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申请/专利号WO2018US56794

发明设计人 LIU CHANG C.;RAVIKUMAR ARJUN;ARZUMANYAN GARRI A.;JAVANPOUR ALEX A.;
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申请日2018-10-19

分类号C12N15/81;C12N15/54;C12N1/18;

国家 WO

入库时间 2022-08-21 11:55:01

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2. 一种利用DNA损伤修复机制使单克隆抗体细胞株进行体内快速进化的新方法 [P] . 中国专利： CN104263755A . 2015-01-07

3. A HIGHLY ERROR-PRONE ORTHOGONAL DNA REPLICATION SYSTEM FOR TARGETED CONTINUOUS EVOLUTION IN VIVO [P] . 世界知识产权组织专利： WO2019079775A2 . 2019-04-25

机译：用于体内有目标连续进化的高度错误消息的正交DNA复制系统

4. ERROR-PRONE DNA POLYMERASE I MUTANTS AND METHODS FOR TARGETED RANDOM MUTAGENESIS IN CONTINUOUS CULTURE USING ERROR-PRONE DNA POLYMERASE I MUTANTS [P] . 欧洲知识产权局专利： EP1504091A4 . 2006-04-26

机译：错字DNA聚合酶I突变以及使用错字DNA聚合酶I突变进行连续培养中目标随机诱变的方法

5. ERROR-PRONE DNA POLYMERASE I MUTANTS AND METHODS FOR TARGETED RANDOM MUTAGENESIS IN CONTINUOUS CULTURE USING ERROR-PRONE DNA POLYMERASE I MUTANTS [P] . 欧洲知识产权局专利： EP1504091A2 . 2005-02-09

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