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METHOD FOR ISOLATING CELL NUCLEI HAVING ENHANCED ANTIGENICITY FROM IMMOBILIZED CELLS OR FFPE TISSUE SECTION, AND ANTIGEN ACTIVATOR AND KIT THEREFOR
METHOD FOR ISOLATING CELL NUCLEI HAVING ENHANCED ANTIGENICITY FROM IMMOBILIZED CELLS OR FFPE TISSUE SECTION, AND ANTIGEN ACTIVATOR AND KIT THEREFOR
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机译:从固定化细胞或FFPE组织切片中分离出具有增强的抗原性的细胞核的方法,以及抗原激活剂和试剂盒
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摘要
Provided are: a pretreatment method for detecting the number of cells containing Ki-67 protein-positive nuclei using Ki-67 antibody; a method for the detection; a kit to be used in the detection method; and determination of a therapy regimen using the aforesaid method. Attempts were made to activate Ki-67 antigen with the use of an enzyme having been considered as inappropriate for the activation thereof. By pretreating a sample with an enzyme not recognizing the epitope of MIB-1 (a rare cutter enzyme), the antigen activation of Ki-67 antigen was enhanced, while enhancing the antigenicity of other antigens including a cytokeratin too. As a result, a method for more objectively and more universally quantifying Ki-67-positive cells at higher reproducibility, said method comprising isolating cell nuclei from an FFPE section while enhancing the antigenicity and performing a reaction between Ki-67 protein, i.e., the target, existing in the cell nuclei with a fluorescently labeled antibody, has been completed.
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