IMAGE-BASED MEASUREMENTS OF FORCE IN A DUAL OPTICAL TRAP

摘要

Optical traps enable nanoscale manipulation of individual biomolecules while measuring molecular forces and lengths. Disclosed herein is a camera-based detection system that enables accurate and precise measurements of forces and interactions in a dual optical trap. Optical traps may be used to stretch and unzip DNA molecules while measuring the displacements of trapped particles from their trapping centers with sub-nanometer accuracy and precision.