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Methods for increasing mutagenic efficiency of genomic sequence modification techniques and molecular complexes used therein
Methods for increasing mutagenic efficiency of genomic sequence modification techniques and molecular complexes used therein
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机译:提高基因组序列修饰技术及其分子复合物诱变效率的方法
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摘要
The present invention relates to a method for producing a double-stranded DNA comprising introducing into a cell containing double-stranded DNA a nucleic acid sequence-recognition module which specifically binds to a target nucleotide sequence in double-stranded DNA and a complex to which PmCDA1 is bound, The target site of the double-stranded DNA, including the step of converting the targeting site, i.e., the target nucleotide sequence and the nucleotide in the vicinity thereof, to another nucleotide, deletion of one or more nucleotides, or insertion of the nucleotide into the site Provides a method of deforming.
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