METHOD AND SET FOR DETECTING MOSQUITO-MEDIATED VIRUS USING MULTIPLEX REAL-TIME POLYMERASE CHAIN REACTION

摘要

The present invention relates to a mosquito-mediated virus detection set and a detection method and, more specifically, to a detection set and method for accurately detecting four kinds of mosquito-mediated virus with high sensitivity by multiplex real-time polymerase chain reaction (RT-PCR). The detection set comprises: (1) a Dengue virus-specific primer pair composed of base sequences of SEQ ID NO: 1 and SEQ ID NO: 2, and a Dengue virus detection probe composed of a base sequence of SEQ ID NO: 3; (2) a Chikungunya virus-specific primer pair composed of base sequences of SEQ ID NO: 4 and SEQ ID NO: 5, and a Chikungunya virus detection probe composed of a base sequence of SEQ ID NO: 6; (3) a yellow fever virus-specific primer pair composed of base sequences of SEQ ID NO: 7 and SEQ ID NO: 8, and a yellow fever virus detection probe composed of a base sequence of SEQ ID NO: 9; and (4) a West Nile virus-specific primer pair composed of base sequences of SEQ ID NO: 10 and SEQ ID NO: 11, and a West Nile virus detection probe composed of a base sequence of SEQ ID NO: 12.;COPYRIGHT KIPO 2019