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IgE Method to diagnose allergy by determination of Immunoglobulin E using an immunoassay based on based on enzyme-mimicking nanozymes
IgE Method to diagnose allergy by determination of Immunoglobulin E using an immunoassay based on based on enzyme-mimicking nanozymes
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机译:基于基于模拟酶的纳米酶的免疫测定,通过测定免疫球蛋白E来诊断过敏的IgE方法
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摘要
The present invention relates to an enzyme-mimicking nanoparticle for allergy diagnosis, a lateral flow immunization color development chip using the same and a manufacturing method thereof, and an immunoplate method and an immunoplate kit for allergy diagnosis using the same. According to an aspect of the present invention, the enzyme-mimicking nanoparticle for allergy diagnosis comprises an inorganic metal catalyst which has a first anti-immunoglobulin E (igE) antibody labeled on a surface thereof by physical binding, is mono-dispersed, and oxidizes a chromogenic substrate. According to the present invention, the lateral flow immunization color development chip for allergy diagnosis and the immunoplate kit for allergy diagnosis use inorganic metal catalyst-based diagnostic immunization with improved variability and instability of enzymes by changes in temperature and pH problematic in conventional enzyme-based diagnostic immunization to increase the diagnostic kit′s stability, shorten test time, and reduce costs in manufacturing, transport, storage, and test processes to improve economic efficiency.
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