Method of preparation of biomimetic hydroxyapatite microspheres

摘要

The present invention relates to a manufacturing method of a biomimetic hydroxyapatite microsphere, and more specifically, to a manufacturing method of biomimetic hydroxyapatite microsphere (HAM) having a size of 0.5-50 μm and comprising the steps of: manufacturing bioactive glass nanoparticles (BGN); immersing the generated bioactive glass nanoparticles in an SBF solution to form bioactive glass aggregates; and mineralizing the formed aggregates into hydroxyapatite. Specifically, the hydroxyapatite microsphere has a porosity and a wide surface area because BGn is obtained by mineralization across the entire as well as a surface. Accordingly, the microsphere has protein loaded thereon through linear (zero-order) and continuous discharges to be used as a drug delivery carrier. In addition, through properties of discharging calcium, phosphate and silicate, the present invention promotes growth of stem cells to be used as a composition for tissue grafts or regeneration promoters. Furthermore, the present invention is not introduced into cells due to a micro-sized structure, and promotes growth of spheroid type stem cell assembly to be used as a composition for tissue grafts or regeneration promotors.