Formulation for Passage and Harvest of Single Cell Human Pluripotent Stem Cells

摘要

The field of the invention relates to cell molecular biology and stem cells. Specifically, the present invention is directed to harvesting and passing single cell stem cells, such as human pluripotent stem cells, comprising: (i) 1 mM to about 30 mM sodium citrate; (ii) a salt comprising 10 mM to 170 mM KCl or NaCl; And (iii) Ca ²⁺ / Mg ²⁺ -free Dulbecco's phosphate buffered saline (DPBS), the formulation comprising about 100 mOsmol / liter to 350 mOsmol / liter of osmolality (osmotic pressure) Has a concentration. This formulation is intended for serial passage and removal of pluripotent stem cells attached to 2D tissue incubators or grown in 3D floating incubators (small and large scale bioreactors), or other stem cells that need to be passaged in the form of single cell populations. Can be used in applications.