TECHNIQUES FOR PREDICTING DETECTING AND REDUCING ASPECIFIC PROTEIN INTERFERENCE IN ASSAYS INVOLVING IMMUNOGLOBULIN SINGLE VARIABLE DOMAINS

摘要

The present invention can be used to predict whether a given ISV will undergo protein interference as described herein and / or generate a (nonspecific) signal in an assay (eg, an ADA immunoassay). (This prediction assay may be used, for example, to test whether a given ISV may have a tendency to generate such protein interference and / or such a signal, and / or to detect the occurrence of such protein interference or such a signal. Can be used to select ISVs that are less prone or less likely to suffer, and / or to test whether some change (s) to the ISVs will reduce (in whole or in part) this interference or its propensity to generate such signals. May be used as an assay or test that may be used and / or to reduce its tendency to generate such protein interferences or signals Can be used as an assay or test that can be used to guide a change or improvement of an ISV to increase); Altering and / or improving the ISV to eliminate or reduce such protein interference or its propensity to generate such signals; Alterations that can be introduced into ISVs, eliminating or reducing such protein interference or its propensity to generate such signals; ISVs specifically selected to not have a tendency to generate such protein interferences or such a signal or to have a (lower) / lower tendency (eg, by using the assay (s) described herein); And altered and / or improved ISVs that do not have a tendency to generate such protein interferences or such signals, or which have a (lower) / lower tendency, and in some specific non-limiting embodiments.