NCL Method for Producing Polypeptide Fragment With High Efficiency Which is Suitable for NCL Method

摘要

[PROBLEMS] To provide a method for efficiently producing a polypeptide fragment suitable for the NCL method. [MEANS FOR SOLVING PROBLEMS] CNBr is reacted with a polypeptide consisting of a first polypeptide fragment and a second polypeptide fragment having cysteine at the N-terminus linked by an intervening sequence of -Cys-W- (His) nZ-Met- A step of obtaining a first polypeptide fragment and a third polypeptide fragment having cysteine at their ends and a step of sequentially reacting a third polypeptide fragment with a compound represented by the formula (I) and a compound represented by the formula (II) To obtain a second polypeptide fragment whose terminal is modified.