dCas9 gRNA Composition and method for improving sensitivity and specificity on detection of nucleic acids with using dCas9 protein and gRNA for binding to target nucleic acid sequence

摘要

The present invention relates to a composition and a method for enhancing the sensitivity and specificity of nucleic acid detection using a dCas9 protein. Based on the functional difference between Cas9 and dCas9, dCas9 and gRNA binding to a target nucleic acid sequence are contained in DNA or RNA When used for nucleic acid amplification, the sensitivity and specificity of target diagnosis can be finally improved by increasing the efficiency of nucleic acid amplification. In particular, when dCas9 and its gRNA are used in a biosensor, the amplification efficiency of the nucleic acid is doubled, the difference between the non-target and the target is increased more than four times, and the non-specific amplification of the non- The composition can be very usefully utilized for improving the sensitivity and specificity of nucleic acid detection.