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A method for simultaneously detecting and identifying Helicobacter pylori and an antibiotics-resistance gene and a use therefor based QuantaMatrix assay Platform
A method for simultaneously detecting and identifying Helicobacter pylori and an antibiotics-resistance gene and a use therefor based QuantaMatrix assay Platform
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机译:同时检测和鉴定幽门螺杆菌和抗生素抗性基因的方法及其基于QuantaMatrix测定平台的用途
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B) isolating the DNA from the sample; PCR amplifying the DNA using the primers of SEQ ID NO: 1 to SEQ ID NO: 8; And c) hybridizing the oligonucleotide probes of SEQ ID NOS: 9 to 23 with the PCR amplification product obtained in step b), and simultaneously identifying the drug resistance gene of Helicobacter pylori. A composition used in the method, and a kit thereof. The present invention relates to a method for identifying a Helicobacter pylori strain based on QMAP, which comprises using 23S rDNA, 16S rDNA, 16S rDNA, which is involved in clarithromycin, Tetracycle, and fluoroquinolones, Gyrase gene resistance was confirmed.
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机译:B)从样品中分离DNA;使用SEQ ID NO:1至SEQ ID NO:8的引物PCR扩增DNA; c)将SEQ ID NO:9至23的寡核苷酸探针与步骤b)中获得的PCR扩增产物杂交,并同时鉴定幽门螺杆菌的耐药基因。该方法中使用的组合物及其试剂盒。本发明涉及基于QMAP的幽门螺杆菌菌株的鉴定方法,该方法包括使用与克拉霉素,四环素和氟喹诺酮类药物有关的23S rDNA,16S rDNA,16S rDNA,以确认回旋酶基因抗性。
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