According to one embodiment of the present invention, there is provided a qRT-PCR primer capable of detecting a hematopoietic cell-based EML4-ALK gene mutation at a detection limit more sensitive than the conventional method. According to another embodiment of the present invention, it is possible to detect EML4-ALK gene mutation using blood circulating lung cancer cells in lung cancer patients in which ALK-FISH can not be performed due to difficulty in collecting solid biopsy of lung cancer tissue, Provides a screening method for lung cancer patients to determine whether anticancer agents targeting gene mutations can be applied to lung cancer patients.
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