Screening methods for sugar isomerizing enzymes using engineered recombinant strains with modified sugar metabolic pathways

摘要

The present invention relates to a recombinant strain having a modified sugar metabolic pathway by introducing enzymes derived from other strains into a strain, and to obtain a useful substance or to screen a variety of variants and variant enzymes capable of producing a useful substance. It is about how it can be. Using the recombinant vector and strain according to the present invention, not only can a new metabolic pathway be constructed in the strain to effectively obtain D-tagatose from D-galactose, but also after introducing a randomly modified sugar converting enzyme. By performing a cell growth-based ultrafast screening method, D-galactose glycoconversion mutase can be screened quickly.