The present invention relates to a mass production method of Astragalus arrhythmia, comprising the steps of: (a) sterilizing Astragalus seeds and aseptically; (b) inducing germination of the sterile yellow seed; (c) inducing root muscle using plant hormones from the germinated Astragalus; (d) liquid culturing the induced root muscles; and (e) treating the cultured root muscles with an elicitor and incubating in a bioreactor, thereby providing an astragaloside IV. A large quantity of Astragalus canopia can be produced with significantly increased content of).
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