PURIFIED EXTRACT (ATS2) SEPARATED FROM PSEUDOLYSIMACHION ROTUNDUM VAR SUBINTEGRUM WITH HIGH CONTENT OF ACTIVE INGREDIENT, ITS PRODUCTION AND COMPOSITION CONTAINING THE ABOVE EXTRACT AS AN ACTIVE INGREDIENT TO PREVENT OR TREAT INFLAMMATION, ALLERGIES AND ASTHMA

摘要

FIELD: pharmaceutical industry.;SUBSTANCE: invention relates to the pharmaceutical industry, in particular to an extract of Pseudolysimachion rotundum var subintegrum for the prevention and treatment of inflammatory disease. Pseudolysimachion rotundum var subintegrum extract for the prevention and treatment of inflammatory diseases, containing 30–60 % (w/w) veprozide, 0.5–10 % (w/w) veratric acid, 2–20 % (w/w) catalposide, 1–10 % (w/w) picroside II, 1–10 % (w/w) isovanyloylcate and 2–20 % (w/w) 6-O-veratroylcatalpol based on the total weight of the extract (100 %) Pseudolysimachion rotundum var subintegrum, where the specified extract obtained by the method, including: adding aqueous ethanol to the dried Pseudolysimachion rotundum var subintegrum at the 1st stage; carrying out the extraction with cold water and then carrying out the extraction under reflux; repeating these steps to obtain the 1st extract in the 2nd stage; suspending said 1st extract in approximately 1–5 times the volume of water to obtain a suspended extract in step 3; adding 1–10 times the volume of butanol, fractionating to obtain an aqueous layer and a butanol layer and collecting the butanol layer to obtain said purified extract, fractionated using butanol (ATS1), at the 3rd stage and purification of the specified purified extract, fractionated using butanol (ATS1), using reverse-phase distribution chromatography using a stationary phase selected from a stationary phase based on silica gel or a stationary phase based on polymer, and a mobile phase selected from the group consisting of water, acetonitrile, methanol, ethanol, butanol, tetrahydrofuran (THF), and mixtures thereof, under certain conditions. Method of obtaining the extract, as well as a pharmaceutical composition.;EFFECT: extract described above has a pronounced anti-inflammatory effect.;3 cl, 15 dwg, 8 ex, 10 tbl