PEPTIDES ANTIOXIDANT ACTIVITY QUANTITATIVE DETERMINATION METHOD

摘要

FIELD: measurement technology.;SUBSTANCE: invention relates to the determinations and tests, in particular to the animal origin peptides antioxidant activity determining method. Animal origin peptides antioxidant activity quantitative determination method includes studied peptides with 2,2-diphenyl-1-picrylhydrazyl (DPPH) in the organic solvent selected from the group including methanol, ethanol, isopropanol, acetone and methyl ethyl ketone reaction product optical density spectrophotometric determination, reduced L-glutathione (GSH) natural origin reference peptide antioxidant with 2,2-diphenyl-1-picrylhydrazyl reaction product optical density determination and peptides under study antioxidant activity calculation as the GSHEAC (GSH equivalent antioxidant capacity) glutathione-equivalent antioxidant activity, which is the ratio of the L-glutathione concentration, at which 50 % of DPPH is reacted to the substance under study concentration, at which 50 % of DPPH is reacted.;EFFECT: proposed is the peptides antioxidant activity quantitative determination method.;1 cl, 4 tbl