METHOD OF MICROSCOPIC DIAGNOSTICS OF SPERM QUALITY AFTER EJACULATE SEDIMENTATION

摘要

FIELD: medicine.;SUBSTANCE: invention relates to the field of medicine and medical diagnostics. Method of microscopic diagnosis of the quality of sperm after sedimentation of the ejaculate, including the selection of cells from the sediment of ejaculate (SE), the preparation of cytological preparations from selected SE cells with placement on slides, is disclosed, drying and staining of preparations, the subsequent count of the number, evaluation of various cell types and comparison with the data of the control group to obtain a conclusion. At the same time, before taking a diagnostic material, they provoke an inflammatory process for the subsequent detection of an infectious agent; SE is obtained by sedimentation of ejaculate; for this purpose, ejaculate is incubated for 20–30 minutes in a thermostat at a temperature of 37 °C until complete liquefaction, the portion is transferred to a graduated centrifuge tube; centrifugation is carried out at 1,000–1,500 rpm for 15–20 minutes; using a pipette, the supernatant is separated without trapping sediment from the bottom; the preparation is prepared using an automatic dosing pipette, which is taken 10 mcl of the prepared SE in such a way that the smear covers an area of 1/3–2/3 of the glass slide, while using slides with high adhesion; drying of the preparation is carried out in a thermostat for 15–20 minutes in conditions of high humidity and at a temperature of 37 °C. Cells are counted and evaluated using a microscope, adjusting its optical magnification.;EFFECT: invention makes it possible to identify malignant findings, to increase the diagnostic value of the biomaterial, to conduct a comprehensive microscopic study and to improve the accuracy of the study.;4 cl, 25 dwg