NON-DESTRUCTIVE METHOD OF ASSESSING CYTOTOXICITY OF NANOPARTICLES USING A MICROALGA DUNALIELLA SALINA AS A BIOSENSOR

摘要

FIELD: measuring equipment.;SUBSTANCE: invention relates to environmental toxicology and biotechnology and is intended for rapid assessment of cytotoxicity of gold nanoparticles. Method for assessing cytotoxicity of gold nanoparticles, comprising non-destructive photometric assessment of chlorophyll content in suspensions of microalgae cultures Dunaliella salina, includes cultivation of microalgae Dunaliella salina using Ben-Amotz nutrient medium, diluting gold nanoparticles with Ben-Amotz culture medium, preparation of samples by introduction of microalgae culture Dunaliella salina into diluted gold nanoparticles so that the sowing dose is 10⁶ cells/ml, triplication of samples, incubation of samples for 48 hours, photometric measurement of suspensions of cultures Dunaliella salina in vivo by recording extinction at three wavelengths: 640, 680 and 740 nm, calculating chlorophyll absorption peak height, calculating toxic action efficiency by formula, calculating values of semi-efficient concentration of gold nanoparticles EC50₄₈ by linear interpolation by formula.;EFFECT: method described above enables to evaluate effectively the toxic effect of gold nanoparticles.;1 cl, 4 dwg, 2 tbl, 2 ex