Method for the preparation and prolonged storage of growth factors and cytokines obtained from platelet rich plasma

摘要

Described is a method of producing a storage stable autologous composition comprising regenerative growth factors and cytokines obtained from platelets. The method comprises the steps of collecting or providing blood from a mammal; delivering the blood to a receptacle; centrifuging the blood to separate a platelet rich plasma component from the blood; collecting the platelet rich plasma component; passing the platelet rich plasma component through a small pore filter to produce the storage stable autologous composition comprising regenerative growth factors and cytokines derived from platelets; and collecting the storage stable autologous composition. The storage stable autologous composition retains regenerative biological activity for up to at least nine months when stored in a frozen state. Also described is a storage stable autologous composition comprising regenerative growth factors and cytokines obtained from platelets produced by the method as described.