Method of producing polyclonal antibodies, and testing methods and apparatus using such antibodies, application to differentiation of gram positive and gram

摘要

A method of producing a polyclonal antibody targeted towards a particular trait of a microorganism, comprising : a) obtaining antigens specific to said trait from multiple different microorganisms expressing said trait; b) mixing said antigens to produce a mixed antigen; c) coupling said mixed antigen to a carrier protein to produce an immunogen; d) injecting said immunogen into an animal; e) obtaining polyclonal antibodies targeted towards said trait from said animal. The trait, may be gram positive and gram negative bacteria, and the antigen teichois acid or lipopolysaccharide. Also disclosed is a lateral flow point of care test device comprising such a polyclonal antibody, as well as a method of use of such a device in detecting microrganisms