The present invention relates to a high-energy composite peptide selenoprotein nutrient solution, preparation method and application thereof. The nutrient solution is composed of selenium-containing materials, proteins, minerals, vitamins and natural energy ores, etc.. Through protein enzymatic treatment, synthesis of peptide selenoprotein complex, energy treatment, complexation reaction and fortification, etc., the high-energy composite peptide selenoprotein nutrient solution containing rich selenium, minerals, vitamins and natural energy substances is prepared, which can be widely used in the development of various kinds of selenium-rich agricultural products (including various kinds of grain crops, vegetables, fruits, fungi, wild plants, sea foods, animal meats, herbs, etc.). Their applications are also disclosed herein. The mother liquor of high-energy composite peptide selenoprotein nutrient solution is diluted with water by 6000 to 8000 times; in the different growing period of crops, irrigate and spray the nutrient solution to the roots and foliages of crops, generally spraying 3-6 times, to get the selenium-rich agricultural products that can meet the standards.
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