PROTEIN STRUCTURE ANALYSIS BASED ON RED-EDGE EXCITATION SHIFT (REES) SPECTROSCOPY

摘要

A protein structure analysis method includes receiving a fluorescence emission spectrum generated by a protein sample at a first excitation wavelength, evaluating, from the fluorescence emission spectrum, an indication characteristic of a fluorescence response of the protein sample at the first excitation wavelength, and repeating the receiving and evaluating steps in relation to a plurality of fluorescence emission spectra generated by the protein sample at a different excitation wavelength to the first excitation wavelength. In addition, the method includes generating a non-linear relationship between excitation wavelength and indication characteristic of fluorescence response of the protein sample that includes an indication of at least one characteristic of the conformational state of the protein sample. The method can be used with any protein which includes one or more naturally occurring fluorescent amino acids (intrinsic fluorophores), and particularly those with Trp residues (most), or proteins having appropriately selected extrinsic fluorophores.