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METHODS FOR PREVENTING DISULFIDE BOND REDUCTION IN CELL CULTURE HARVEST
METHODS FOR PREVENTING DISULFIDE BOND REDUCTION IN CELL CULTURE HARVEST
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机译:防止细胞培养收获中二硫键还原的方法
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摘要
This disclosure relates to methods for the prevention of the reduction of disulfide bonds in a polypeptide expressed in a recombinant host cell, comprising, following fermentation, adding phosphate to a harvest solution of the recombinant host cell, wherein the disulfide bond in the polypeptide remains non-reduced.
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