首页> 外国专利> A METHOD TO BUILD FUNGAL PRODUCTION STRAINS USING AUTOMATED STEPS FOR GENETIC MANIPULATION AND STRAIN PURIFICATION

A METHOD TO BUILD FUNGAL PRODUCTION STRAINS USING AUTOMATED STEPS FOR GENETIC MANIPULATION AND STRAIN PURIFICATION

机译:利用自动化步骤进行遗传操纵和纯化的真菌生产菌株的构建方法

摘要

The present disclosure provides a high-throughput (HTP) microbial genomic engineering method and system for transforming, screening, and selecting filamentous fungal cells that utilizes automation. The method and system utilize HTP selection and counter-selection to purify homokaryotic transformed filamentous fungal cells. Furthermore, the present disclosure provides a method for producing and long-term storage of protoplasts derived from filamentous fungal cells.
机译:本公开提供了利用自动化的用于转化,筛选和选择丝状真菌细胞的高通量(HTP)微生物基因组工程方法和系统。该方法和系统利用HTP选择和反选择来纯化同核转化的丝状真菌细胞。此外,本公开提供了一种用于产生和长期储存源自丝状真菌细胞的原生质体的方法。

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