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A METHOD TO BUILD FUNGAL PRODUCTION STRAINS USING AUTOMATED STEPS FOR GENETIC MANIPULATION AND STRAIN PURIFICATION
A METHOD TO BUILD FUNGAL PRODUCTION STRAINS USING AUTOMATED STEPS FOR GENETIC MANIPULATION AND STRAIN PURIFICATION
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机译:利用自动化步骤进行遗传操纵和纯化的真菌生产菌株的构建方法
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摘要
The present disclosure provides a high-throughput (HTP) microbial genomic engineering method and system for transforming, screening, and selecting filamentous fungal cells that utilizes automation. The method and system utilize HTP selection and counter-selection to purify homokaryotic transformed filamentous fungal cells. Furthermore, the present disclosure provides a method for producing and long-term storage of protoplasts derived from filamentous fungal cells.
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