首页> 外国专利> SRM/MRM ASSAY FOR THE TYROSINE-PROTEIN KINASE RECEPTOR UFO (AXL) PROTEIN

SRM/MRM ASSAY FOR THE TYROSINE-PROTEIN KINASE RECEPTOR UFO (AXL) PROTEIN

机译:酪氨酸蛋白激酶受体UFO(AXL)蛋白的SRM / MRM分析

摘要

Peptides from the tyrosine-protein kinase receptor UFO protein (AXL) are provided that are particularly advantageous for quantifying the AXL protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed and include formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks. A protein digest is prepared from the biological sample and the AXL protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described.
机译:提供了来自酪氨酸蛋白激酶受体UFO蛋白(AXL)的肽,这些肽对于通过选择反应监测(SRM)/多反应监测(MRM)方法在福尔马林固定的生物样品中直接定量AXL蛋白特别有利。 ) 质谱。此类生物样品经过化学保存和固定,包括福尔马林固定的组织/细胞,福尔马林固定/石蜡包埋(FFPE)组织/细胞,FFPE组织块和来自那些块的细胞。从生物样品制备蛋白质消化物,并通过SRM / MRM质谱法通过在蛋白质样品中定量描述的至少一种或多种肽,在液体组织样品中定量AXL蛋白。

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