首页>
外国专利>
METHOD OF APTAMER SELECTION AND METHOD FOR PURIFYING BIOMOLECULES WITH APTAMERS
METHOD OF APTAMER SELECTION AND METHOD FOR PURIFYING BIOMOLECULES WITH APTAMERS
展开▼
机译:适体选择方法和带有适体的生物分子纯化方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention proposes a method of using oligonucleotide aptamers binding with certain specific biomolecules associated with a disease to purify the specific biomolecules from disease patient's samples and to identify biomarkers for the particular disease. The present invention describes a method using the magnetic assisted rapid aptamer selection (MARAS) to select aptamers having high binding affinity for certain disease-related biomolecules and not for other non-disease-related biomolecules from nucleic acid libraries. Meanwhile, the present invention also proposes a method of using the aptamer obtained above as a capture ligand to purify certain specific biomolecules related to a disease from disease patients' samples. Further, the present invention describes the use of the obtained aptamer as the capture ligand to purify biomolecules with a specific binding affinity range by applying an oscillating magnetic field range as a virtual filter.
展开▼