METHOD FOR PRODUCING LIBRARY FOR USE IN PREDICTION OF ACTION OF SUBSTANCE OF INTEREST ON LIVING BODY, AND METHOD FOR EVALUATING ACTION OF SUBSTANCE OF INTEREST ON LIVING BODY BASED ON CHANGE IN ACTION PARAMETER OR THE LIKE OF ZEBRAFISH

摘要

A method for producing a library for use in prediction of the action of a substance of interest on the living organism, the library having a large number of numerical data obtained based on change in a behavioral parameter of zebrafish and a large number of numerical data representing change in expression levels of genes integrated and recorded therein, the method including the steps of: preparing a substance of known action that is a substance whose action on the living organism is known; measuring a behavioral parameter of zebrafish not given the substance of known action in a bath having the zebrafish therein; administering the substance of known action to the bath having the zebrafish therein and measuring the behavioral parameter of the zebrafish after the administration of the substance of known action in the bath having the zebrafish therein; detecting change in the behavioral parameter of the zebrafish by the administration of the substance of known action; subdividing data representing the change in the obtained behavioral parameter and converting the data into a large number of numerical data; normalizing the large number of numerical data obtained based on the change in the behavioral parameter; normalizing a large number of numerical data representing the change in expression levels of genes after the administration of the substance of known action; and integrating and recording the large number of numerical data obtained based on the change in the behavioral parameter and the large number of numerical data representing change in expression levels of genes, both normalized.