A PROCESS FOR BIO-SLUDGE REDUCTION IN HYDROCARBON REFINERY EFFLUENT TREATMENT PLANT THROUGH MICROBIAL INTERVENTIONS

摘要

A process for treatment of hydrocarbon refinery wastewater producing a low bio-sludge and said process comprises utilizing microbial consortia comprising at least one species of Pseudomonas and at least one species of Bacillus in a ratio of 10:1 to 1:10, wherein species of Pseudomonas and species of Bacillus have constitutive expression of at least one hydrocarbon degrading gene, wherein the species of Pseudomonas are selected from the group consisting of Pseudomonas stutzeri (MTCC 25027), Pseudomonas aeruginosa (MTCC 5389), Pseudomonas aeruginosa strain IOC DHT (MTCC, 5385), Pseudomonas putida IOCRI (MTCC 5387), Pseudomonas putida IOC5al (MTCC 5388) and a mutant thereof, wherein the species of Bacillus are selected from the group consisting of Bacillus subtilis (MTCC 25026), Bacillus substilis (MTCC 5386), Bacillus thermoleovorans (MTCC 25023), Bacillus stearothermophilus (MTCC 25030), Lysinibacillus sp. (MTCC 25029), Lysinibacillus sp. (MTCC 5666) and a mutant thereof, and wherein the microbial consortia is used in concentration of at least 10² cfu/ml.