METHODS FOR IN VITRO SITE-DIRECTED MUTAGENESIS USING GENE EDITING TECHNOLOGIES

摘要

The invention relates to methods for performing in vitro site-directed mutagenesis of a targeted gene or genes. In another aspect, the invention includes in vitro site-directed mutagenesis kits comprising a ribonucleotide particle (RNP), an oligonucleotide, a buffer, a cell-free extract, and instructional material for use thereof.