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MATERIALS AND METHODS FOR ALKENE REDUCTION OF LEVOGLUCOSENONE BY AN ALKENE REDUCTASE
MATERIALS AND METHODS FOR ALKENE REDUCTION OF LEVOGLUCOSENONE BY AN ALKENE REDUCTASE
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机译:烯烃还原酶还原左旋糖皮质激素的烯烃的材料和方法
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摘要
The subject invention concerns materials and methods for alkene reduction of compounds, such as levoglucosenone (LGO) and (,S)-Y-hydroxymethyl-a,P-butenolide (HBO), using an alkene reductase enzyme. In one embodiment, a method of the invention comprises alkene reduction of a target compound by reacting the compound with an Old Yellow Enzyme (OYE) that reduces alkene bonds. In one embodiment, the OYE is OYE 2.6 from Pichia stipites and comprises the amino acid sequence of SEQ ID NO: 1. In a specific embodiment, the enzyme is an Old Yellow Enzyme (OYE) 2.6 mutant having an amino acid substitution at position 78 in the sequence, wherein the tyrosine at position 78 is substituted with a tryptophan amino acid (Y78W) and is designated as OYE 2.6 Y78W (SEQ ID NO:2).
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机译:本发明涉及用于使用烯烃还原酶将化合物例如左葡糖醛酮(LGO)和(,S)-Y-羟甲基-α,P-丁烯内酯(HBO)的烯烃还原的材料和方法。在一个实施方案中,本发明的方法包括通过使目标化合物与还原烯键的老黄酶(OYE)反应来使目标化合物烯还原。在一个实施方案中,OYE是来自毕赤酵母的OYE 2.6,并包含SEQ ID NO:1的氨基酸序列。在一个具体的实施方案中,该酶是在位置78具有氨基酸取代的老黄酶(OYE)2.6突变体。在该序列中,其中第78位的酪氨酸被色氨酸(Y78W)取代,并被命名为OYE 2.6 Y78W(SEQ ID NO:2)。
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