CELL-FREE SYNTHESIS OF ISOTOPIC LABELLED PROTEINS FROM AMINO-ACIDS PRECURSORS

摘要

Process of cell-free synthesis of a target protein, comprising at least the following steps: a. providing at least one precursor of an amino-acid, a cell-free extract comprising means for transforming said precursor of an amino-acid into the corresponding amino-acid, at least one tRNA for integrating said amino-acid into the corresponding target protein, in which the at least one precursor of amino-acid is an alpha-keto acid, with the proviso that 2-ketobutyric acid is excluded, b. mixing the compounds provided in step a) to obtain a mixture, c. adding to said mixture obtained in step b) a DNA or a mRNA coding to said target protein in order to produce said target protein.