METHOD OF DETECTING MICROORGANISM IN SAMPLE BY FLUORESCENCE BASED DETECTION METHOD

摘要

To provide a reagent suited for detecting a microorganism in a complex sample.SOLUTION: The present invention relates to a method of detecting a microorganism, in particular Staphylococcus aureus, in a sample, comprising the steps of a) incubating the sample with a slow off-rate modified aptamer (SOMAmer) comprising a fluorescent label, b) optionally washing the sample, c) analyzing the sample by a fluorescence-based detection method. The invention further relates to a SOMAmer comprising a fluorescent label (where, the SOMAmer comprises a nucleotide sequence specific for Staphylococcus aureus), its use for detecting Staphylococcus aureus cells in a sample, and to a microarray or biosensor and a kit comprising at least one of such SOMAmers comprising a fluorescent label.SELECTED DRAWING: Figure 1