Methods for Providing Chimeric Antigen Receptors (CARs) to Individualized Cells

摘要

PROBLEM TO BE SOLVED: To provide a method for selecting an antigen specific to a cancer cell, which is not expressed in a healthy tissue, in order to design a cell that kills / lysess the cancer cell without attacking the non-tumor cell. To provide a cell sample containing tumor cells and non-tumor cells, and to bring the cell tissue into contact with a conjugate containing a fluorescent moiety and an antigen-recognizing moiety (hereinafter referred to as "conjugate"), binding from the cell tissue. By repeating the steps of taking out the unbonded conjugate and detecting the cells bound to the conjugate by the fluorescent radiation emitted by the fluorescent portion of the first conjugate, and extinguishing the fluorescent radiation emitted by the fluorescent portion of the conjugate. No method for providing cells containing chimeric antigen receptors (CARs) specific for one or more target antigens exposed in tumor cells characterized.