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PROTEIN TARGET NUCLEIC ACID APTAMER, METHOD FOR PRODUCING THE SAME, AND METHOD FOR DETECTING TARGET PROTEIN
PROTEIN TARGET NUCLEIC ACID APTAMER, METHOD FOR PRODUCING THE SAME, AND METHOD FOR DETECTING TARGET PROTEIN
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机译:蛋白质靶核酸核酸适体,其制备方法和靶蛋白质的检测方法
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摘要
To provide a protein target nucleic acid aptamer allowing for detection of a target protein in a living cell, and a method for detecting a target protein.SOLUTION: The protein target nucleic acid aptamer includes: a first oligonucleotide that forms a stem-loop structure and recognizes a fluorescence quencher; a second oligonucleotide located 5' side of the first oligonucleotide; and a third oligonucleotide located on the 3' side of the first oligonucleotide, the second oligonucleotide and the third oligonucleotide cooperates to recognize a target protein. The method for detecting a target protein includes contacting the protein target nucleic acid aptamer with the target protein in the presence of a fluorescent probe including a fluorescent quencher.SELECTED DRAWING: Figure 1
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