METHOD AND COMPOSITION FOR QUICK MULTIPLEX AMPLIFICATION OF STR GENE LOCUS

摘要

To provide a method for amplifying multiplex polymerase chain reaction (PCR) of a short tandem repeat (STR) gene locus that can be used for quickly generating a highly specific STR profile from target nucleic acid.SOLUTION: A method for detecting the presence of at least eight different single-nucleotide polymorphisms (SNPs) in a sample containing nucleic acid, in which the SNPs are detected by: (a) bringing the sample into contact with at least eight unlabeled primers in solution under the condition that hybridization between the primers and the nucleic acid in the sample is enabled; (b) performing labeling by a primer extension reaction, with one of at least eight different fluorescent dyes; and (c) detecting the primer extension product by a laser-induced fluorescence method by using a galvanometer of a step steering mode and a spectroscope equipped with a dispersion diffraction grating and a linear array detector.SELECTED DRAWING: None