Stepwise sequencing with unlabeled reversible terminators or natural nucleotides

摘要

The present invention provides compositions and methods for nucleic acid sequencing and other applications. In sequencing bi-synthesis, an unlabeled reversible terminator is incorporated by the polymerase at each cycle, and is labeled after incorporation by directly or indirectly attaching a labeled antibody or other affinity reagent to the reversible terminator. . [Selection diagram] FIG.